Journal: Data in Brief

Article Title: Mechanical, water contact angle and fiber thickness data for Insulin-like growth gactor-1 (IGF-1) incorporated in electrospun random DegraPol Ⓡ fibers and IGF-1 impact on tenocyte aspect ratio and gene expression data

doi: 10.1016/j.dib.2024.111139

Figure Lengend Snippet:

Article Snippet: Data collection , Scanning electron microscopy (SEM) : A SEM was used (Zeiss Gemini SEM 450) and images of the outer and the inner surfaces of electrospun meshes were used to assess the fiber thickness. Mechanics : For the mechanical assessment, a uniaxial tensile testing machine (Zwick Z010 with a 20 N load cell) was used. Water contact angles (WCA) : Static and dynamic WCA on the material surfaces were measured by a video-based optical contact angle measuring instrument OCA 35 Dataphysics, Germany. qPCR : Rabbit Achilles tenocytes were cultured as monolayers and RNA was extracted by an RNeasy Plus Mini Kit from Qiagen, Hilden, Germany; followed by reverse transcription with the iScript Advanced cDNA Synthesis Kit (Bio-Rad, Cressier, Switzerland). The quantitative real-time PCR reactions were performed with CFX Connect Real-Time PCR Detection System (Bio-Rad, Cressier, Switzerland) and SsoAdvanced SYBR Green Supermix (Bio-Rad, Cressier, Switzerland). Aspect ratio : A microscope was utilized to analyze the cell length and width (Leica Microscope 6000 B, Leica Camera AG, Germany)..

Techniques: Emulsion, Electron Microscopy, Cell Culture, Isolation, Gene Expression, Control, Standard Deviation, Real-time Polymerase Chain Reaction, Reverse Transcription, cDNA Synthesis, SYBR Green Assay, Microscopy

Journal: Data in Brief

Article Title: Mechanical, water contact angle and fiber thickness data for Insulin-like growth gactor-1 (IGF-1) incorporated in electrospun random DegraPol Ⓡ fibers and IGF-1 impact on tenocyte aspect ratio and gene expression data

doi: 10.1016/j.dib.2024.111139

Figure Lengend Snippet:

Article Snippet: Data collection , Scanning electron microscopy (SEM) : A SEM was used (Zeiss Gemini SEM 450) and images of the outer and the inner surfaces of electrospun meshes were used to assess the fiber thickness. Mechanics : For the mechanical assessment, a uniaxial tensile testing machine (Zwick Z010 with a 20 N load cell) was used. Water contact angles (WCA) : Static and dynamic WCA on the material surfaces were measured by a video-based optical contact angle measuring instrument OCA 35 Dataphysics, Germany. qPCR : Rabbit Achilles tenocytes were cultured as monolayers and RNA was extracted by an RNeasy Plus Mini Kit from Qiagen, Hilden, Germany; followed by reverse transcription with the iScript Advanced cDNA Synthesis Kit (Bio-Rad, Cressier, Switzerland). The quantitative real-time PCR reactions were performed with CFX Connect Real-Time PCR Detection System (Bio-Rad, Cressier, Switzerland) and SsoAdvanced SYBR Green Supermix (Bio-Rad, Cressier, Switzerland). Aspect ratio : A microscope was utilized to analyze the cell length and width (Leica Microscope 6000 B, Leica Camera AG, Germany)..

Techniques: Emulsion, Electron Microscopy, Cell Culture, Isolation, Gene Expression, Control, Standard Deviation, Real-time Polymerase Chain Reaction, Reverse Transcription, cDNA Synthesis, SYBR Green Assay, Microscopy